Formalin-fixed paraffin-embedded (FFPE) preservation remains a widely adopted method for long-term storage of clinical specimens, offering cost-effective sample archiving compatible with next-generation sequencing (NGS) applications in both translational research and molecular diagnostics. Among NGS approaches, RNA sequencing (RNA-seq) enables transcriptome-wide profiling, including quantification of gene expression and detection of alternative splicing events. In this study, we evaluated the performance of the fully automated Watchmaker RNA Library Prep Kit with Polaris® Depletion for whole-transcriptome library construction from low-input, degraded FFPE RNA using the Beckman i7 automation platform.
