Cell counts and viability are determined using a Nexcelom Cellometer® with acridine orange and propidium iodide to identify live and dead nucleated cells, respectively. Unlike dissociated tissue, which is prone to cellular debris, PBMCs can be counted using trypan blue exclusion. If flow cytometry is used to determine cell count and viability, it is recommended that proper FSC thresholds be established to remove platelets, which are present in cryopreserved PBMCs.
Colorectal and Lung Tumoroids are Now Available
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Colorectal and Lung Tumoroids are Now Available
NEW Blog Post - Advancing CAR-NK Therapy: Why Leukopak Quality and Donor Characterization Matter
Discovery Partners with PathAI to Develop First AI-Powered Biospecimen Solutions
NEW Blog Post - Leverage Permeabilized Hepatocytes for Difficult Drug Compounds in Metabolism Studies
Discovery Announces Upcoming Launch of Tumoroids at Precision Med Tri-Con