Costimulatory and coinhibitory receptors on T cells are key mediators of the overall immune response, and the clinical success of checkpoint inhibitor therapies has proven the therapeutic benefits of targeting these pathways Viable, cryopreserved dissociated tissue provides an opportunity to understand the dynamic expression patterns of these receptor/lig and sets at the single cell level, providing insights into the differential expression of each receptor/ligand pair across different cancer indications as a means to target new therapeutic interventions.