Immune cell phenotyping of the tumor microenvironment is transforming cancer diagnostics and treatment by identifying novel predictive biomarkers for immunotherapy and powerful prognostic markers, thus providing deeper insights of disease mechanisms. Using our unique, clinical trial-ready multiplexed immunofluorescence (mIF) workflow, we demonstrate how custom mIF and digital image analysis offer an innovative method to investigate invasion in solid tumors by studying the tumor and the corresponding microenvironment.
