Conduct transporter interaction studies faster with Discovery’s solute carrier (SLC) transporter expressing cells and ATP-binding cassette (ABC) transporter vesicles
Gentest TransportoCells™ products are high-performance mammalian cells in a convenient, cryopreserved format that transiently overexpress a single human SLC transporter protein. These frozen cells deliver robust data, while eliminating the time required to culture and maintain stable cell lines.
Options: Transporter-qualified primary hepatocytes are also available for suspension or monolayer assays
Solute carrier (SLC) transporters are membrane proteins involved in drug disposition, and they are responsible for the transport of endogenous and exogenous compounds across biological membranes, making them the ideal model for ADME studies.
Improve the predictive potential of your transporter drug-drug interaction, reaction phenotyping, and toxicity studies with a validated, more human-like transporter-protein expressing vesicle model.
Transporter membrane and vesicle kits reduce the time needed to prepare reagents. The ATPase kit contains the required buffers and chemicals for performing five 96-well microplate assays. The BSEP and BCRP/MRP kits contain all necessary buffers and chemicals for performing 200 assays.
Note: Kits do not contain vesicle or membrane preps.
Gentest TransportoCells™ products are cryopreserved HEK-293 cells transiently overexpressing human SLC drug transporter proteins. Each TransportoCells™ product contains an individually expressed SLC transporter.
The SLC transporters are OATP1B1, OATP1B3, OAT1, OAT3, OCT1, OCT2, MATE1, MATE2-K, PEPT1, PEPT2, NTCP, OATP2B1, OAT2, OAT4, OCTN2, and corresponding Control.
The 2024 The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidance recommends testing OATP1B1, OATP1B3, OAT1, OAT3, OCT2, and MATE1, MATE2-K. These transporters are available as TransportoCells™ products.
NOTE: Additional transporters may be investigated on a case-by-case basis.
Control cells, which are parent HEK-293 cells transiently expressing empty expression vector, are available (Cat. No. 354854). We recommend that negative control cells be tested side-by-side with TransportoCells™ products.
Yes, all TransportoCells™ products are subcloned in the same expression vector as the control cells. respectively, per the recommendations provided by Nexcelom. Given the cellular debris that can be present, we do not recommend the use of trypan blue-based cell counting methods, as this will overestimate the number of dead cells.
TransportoCells™ products are cryopreserved cells in a 2 mL cryogenic vial. The products are shipped in a liquid nitrogen dry shipper. Upon arrival in your lab, the vials should be removed immediately from the dry shipper and placed in the vapor phase of a liquid nitrogen storage tank. The empty dry shipper should be returned following
Typical assays that can be performed with TransportoCells™ products include:
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